Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea
1 Department of Biology, Wilfrid Laurier University, 75 University Avenue W., Waterloo, N2L 3C5, Ontario, Canada
2 Department of Molecular Biology, Centre for Carbohydrate Recognition and Signalling, Aarhus University, Gustav Wields Vej 10, Aarhus C -8000 Denmark
Plant Methods 2011, 7:46 doi:10.1186/1746-4811-7-46Published: 15 December 2011
Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses.