Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.
- Brian G Forde, Lancaster University
Grafting is an extremely important technique for the study of long distance signalling in plants. The authors describe substantial improvements to existing methodologies that both increase the success rate and the throughput of hypocotyl grafting in Arabidopsis.
Hydroponics is an extremely useful technique for growing plants under controlled nutrient conditions, particularly where clean roots are needed for physiological or microscopic analysis or for RNA extraction. The detailed protocol set out here should make it straightforward for other laboratories to rapidly and successfully adopt the technique. A video is also available at http://t.co/WoAYgYy84C.
The authors present a detailed series of image analysis tools for the detection and measurement in plant tissues of callose deposition, an important defence against fungal pathogens. The software should be very useful for researchers interested in quantifying callose-mediated defences against plant pathogens. In addition, automated analysis of the progress of callose development over time can be used to track fungal development within the leaf.
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- Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii http://t.co/xl17fHCrG3 2:14 PM May 12th, 2013
- A new 96-well microphenotyping method is designed for chemical genetic approaches to the study of root development http://t.co/tabeIAxVQx 9:06 PM May 4th, 2013
- Plant Methods paper describes improvements to the tricky technique of hypocotyl grafting in Arabidopsis http://t.co/aQnvEyRbwv 8:51 PM May 4th, 2013
Brian G. Forde is currently Professor of Environmental Plant Biotechnology at the Lancaster Environment Centre, Lancaster University, in the UK.
“Technological innovation is probably the most important catalyst for progress in any scientific discipline. When we launched Plant Methods in 2005 our aim was to provide a high-profile, Open Access platform to stimulate the development and dissemination of new and improved techniques and research tools in plant biology. Just seven years later we have published over 200 papers describing new techniques or resources of value to the plant science community and our Thomson Reuters Impact Factor of 2.83 puts us amongst the top 20% of plant journals”
Brian G. Forde
Hydroponics: a step-by-step guide for plant researchers
Plant Methods 2013, 9:4
Example uses of Cell-o-Tape, which semi-automates the measurement and counting of cells and can estimate the locations of feature points.
Plant Methods 2012, 8:7
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