Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.
- Brian G Forde, Lancaster University
Targeting Induced Local Lesions IN Genomes (TILLING) is a process for high-throughput screening of mutagenised populations for mutations in genes of interest. TILLING has proven a useful tool for plant biologists, but the development of mutant populations is a lengthy process, often requiring at least two full growing seasons before a new population can be assessed. Serrat et. al., describe a new method for obtaining rice TILLING mutants based on seed-derived callus culture, that circumvents the need for growing two successive generations when developing a novel population.
Terpenoids are of great importance for human nutrition, in the fragrance industry and in human medicine. This paper describes a method for the functional characterisation of genes involved in diterpene biosynthesis using an optimized transient expression assay in Nicotiana benthamiana leaves. The method could have applications in both the reconstitution and the metabolic engineering of diterpenoid biosynthetic pathways in plants.
The CRISPR/Cas system is an exciting new technology that provides a simplified and effective way to achieve the targeted editing of complex genomes. The first reports of its application to plants have only appeared in the past two months. In their review of a rapidly moving field the authors summarize and discuss the current status of CRISPR/Cas technology in plant research.
Brian G. Forde is currently Professor of Environmental Plant Biotechnology at the Lancaster Environment Centre, Lancaster University, in the UK.
“We launched Plant Methods in 2005 with the aim of providing a high-profile, open access platform that would help to stimulate the development and dissemination of novel techniques and research tools in plant biology.
By the end of 2013 we had published over 270 papers, which have now collectively been cited over 2500 times. Remarkably, the 100 most accessed of these have been viewed online a total of more than 1.5 million times - most visits coming from the US, but with India and China close behind in 2nd and 3rd places, respectively.
These figures are encouraging evidence that Plant Methods is achieving its goals of providing international impact for our authors and open access for the plant science community to a diverse and ever-expanding range of innovative techniques and resources. To keep up to date with what Plant Methods is publishing - and other news - why not sign up to our Twitter feed using the link below?”
Brian G. Forde
Hydroponics: a step-by-step guide for plant researchers
Plant Methods 2013, 9:4
High throughput quantitative phenotyping of plant resistance using chlorophyll fluorescence image analysis
Plant Methods 2013, 9:17
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