|
Medicago reference genes and primers for qRT-PCR |
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| Gene Name |
TC |
Accession Number |
Forward/Reverse Primer (5'-3') |
PCR Product Size (bp) |
PCR efficiency (E) |
R2 |
|
|
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| β Tubulin |
TC106341 |
N |
TTTGCTCCTCTTACATCCCGTG / GCAGCACACATCATGTTTTTGG |
100 |
1.08 |
1.00 |
| PPRrep |
TC96273 |
N |
GGAAAACTGGAGGATGCACGTA / ACAAGCCCTCGACACAAAACC |
100 |
0.93 |
1.00 |
| PDF 2 |
TC107161 |
N |
GTGTTTTGCTTCCGCCGTT / CCAAATCTTGCTCCCTCATCTG |
100 |
0.99 |
1.00 |
| bHLH |
CX538576 |
N |
TAGCGAGTACCATGATGCCAGA / GCGCCTCTTTTGTTTTCAGC |
100 |
0.89 |
1.00 |
| UBC |
AW686873 |
N |
CTGACAGCCCACTGAATTGTGA / TTTTGGCATTGCTGCAAGC |
100 |
0.96 |
1.00 |
| PTB |
TC111751 |
N |
CGCCTTGTCAGCATTGATGTC / TGAACCAGTGCCTGGAATCCT |
100 |
0.85 |
1.00 |
| Ubiquitin |
TC102473 |
AC137828_19.4 |
GCAGATAGACACGCTGGGA / AACTCTTGGGCAGGCAATAA |
100 |
0.95 |
1.00 |
| UBC9 |
TC106312 |
AC137602_2.4 |
GGTTGATTGCTCTTCTCTCCCC / AAGTGATTGCTCGTCCAACCC |
100 |
1.13 |
0.99 |
| Helicase |
CB892427 |
N |
GTACGAGGTCGGTGCTCTTGAA / GCAACCGAAAATTGCACCATAC |
100 |
0.91 |
1.00 |
| ELF1α |
EST317575 |
N |
GACAAGCGTGTGATCGAGAGATT / TTTCACGCTCAGCCTTAAGCT |
100 |
0.68 |
0.98 |
| UPL7 |
TC111218 |
N |
CCAGTTGTTCTCGTGGTCCATT / CCTCCAATTGTCGCCCAAA |
100 |
0.93 |
1.00 |
| GAPDH |
MtC00030_GC |
CT573421_3.4 |
TGCCTACCGTCGATGTTTCAGT / TTGCCCTCTGATTCCTCCTTG |
100 |
1.04 |
0.99 |
| Actin2 |
TC107326 |
AC137836_27.5 |
TCAATGTGCCTGCCATGTATGT / ACTCACACCGTCACCAGAATCC |
100 |
1.12 |
0.99 |
| MSC27 |
X63872 (M. sativa) |
N |
GTTGAAGTAGACATTGGTGCTAACG / AGCTGAGTCATCAACACCCTCAT |
100 |
0.76 |
0.99 |
|
Mean PCR efficiency (E) was determined from three biological replicates of each of six organs, using LinRegPCR [31], which also yielded mean R2. N, no corresponding GenBank accession number. | ||||||
Kakar et al. Plant Methods 2008 4:18 doi:10.1186/1746-4811-4-18 |
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