Additional file 1.

Additional examples for the specificity of SYTOX dyes for non-viable cells. Shown are roots stained with fluorescein diacetate (cyan) and SYTOX orange (red). (A), (B) Detail of root showing cells at the end of the lateral root cap. (A) Fluorescein diacetate fluorescence. (B) Overlay of fluorescein diacetate and SYTOX orange fluorescence. While a SYTOX orange stained cell is seen in (B), a gap can be seen in (A) at the same position. (C). Confocal optical section through a root showing long cells at the end of the lateral root cap being selectively stained with SYTOX orange. Scalebars: 50 μm.

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Truernit et al. Plant Methods 2008 4:15   doi:10.1186/1746-4811-4-15