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Controls for ChIP and QPCR |
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| Controls |
Primers sets to test |
Purpose |
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| (a) Controls for ChIP |
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| Input Sample |
All primer sets |
Positive control for presence of chromatin, used to calculate %IP |
| No Antibody control |
All primer sets |
Determines background signal level |
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| (b) Controls for QPCR |
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| Calibration line |
All primer sets |
Positive control for QPCR, used for quantification of QPCR signals |
| Melting curve |
All primer sets |
Tests for amplification of the correct fragment |
| No-template PCR |
All primer sets |
Negative control for QPCR, indicates primer artifacts |
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| (c) Controls for data interpretation |
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| ChIP control (+) |
Control gene primer set |
Positive control for ChIP (e.g. actin for H3K4me2) |
| ChIP control (-) |
Control gene primer set |
Negative control for ChIP (e.g. actin for H3K9me2) |
Haring et al. Plant Methods 2007 3:11 doi:10.1186/1746-4811-3-11 |
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