Figure 3.

Use of the Sucrose Prep to identify T-DNA insertion knock-out mutations. Putative mutant plants homozygous for the T-DNA insertion were identified with the Sucrose Prep using gene specific primers for AtWRKY22 (Table 2) and amplification of DNA by PCR. The lines were also tested by RT-PCR for the loss of AtWRKY22 transcript using a commercial RNA isolation kit (Qiagen). Positions of the primers used for amplification are indicated below the schematic diagram.