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		Methodology A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction Jana Svacinova, Ondrej Novak, Lenka Plackova, Rene Lenobel, Josef Holik, Miroslav Strnad, Karel Dolezal Plant Methods 2012, 8:17 (17 May 2012) Abstract \| Provisional PDF \| Editor’s summary A microscale high-throughput method for the analysis of the cytokinin content of plant tissues.
		Methodology The Arabidopsis thaliana - Alternaria brassicicola pathosystem: a model interaction for investigating seed transmission of necrotrophic fungi. Stephanie Pochon, Emmanuel Terrasson, Thomas Guillemette, Beatrice Iacomi-Vasilescu, Sonia Georgeault, Marjorie Juchaux, Romain Berruyer, Isabelle Debeaujon, Philippe Simoneau, Claire Campion Plant Methods 2012, 8:16 (9 May 2012) Abstract \| Provisional PDF \| PubMed
		Methodology Protocol: optimised electrophysiological analysis of intact guard cells from Arabidopsis Zhong-Hua Chen, Cornelia Eisenach, Xin-Qin Xu, Adrian Hills, Michael R. Blatt Plant Methods 2012, 8:15 (6 May 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Guard cells are functionally important in their role regulating stomatal aperture and offer many advantages for electrophysiological analysis. The detailed protocols for voltage clamp analysis of Arabidopsis guard cells set out here allow measurements of much longer duration than was previously possible.
		Methodology Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies Andrea Pitzschke, Helene Persak Plant Methods 2012, 8:14 (4 May 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Transient expression is an extremely valuable component of the molecular biologist's toolbox. The authors describe a new version of this tool that promises to make the procedure more reliable - as well as being accessible to more laboratories.
		Methodology A novel fluorescent assay for sucrose transporters Peter J Gora, Anke Reinders, John M Ward Plant Methods 2012, 8:13 (4 April 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Budding yeast is an important model system for the heterologous expression of plant sucrose transporters. The authors have exploited the ability of type I sucrose uptake transporters to transport the fluorescent β-glucoside, esculin, to develop a rapid and sensitive assay system that should be a valuable tool for the functional analysis of members of this sucrose transporter family.
		Methodology Targeted parallel sequencing of large genetically-defined genomic regions for identifying mutations in Arabidopsis Kun-hsiang Liu, Matthew McCormack, Jen Sheen Plant Methods 2012, 8:12 (30 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Next generation sequencing (NGS) is becoming a powerful tool for identifying mutations at the molecular level. This paper describes an innovation that has the potential to make the NGS approach accessible to many more laboratories. In the targeted parallel sequencing (TPSeq) method only genetically defined genomic regions are sequenced, making the process less expensive and the downstream bioinformatics more straightforward.
		Methodology Xylem sap collection and extraction methodologies to determine in vivo concentrations of ABA and its bound forms by gas chromatography-mass spectrometry (GC-MS) Andrew G Netting, Julian C Theobald, Ian C Dodd Plant Methods 2012, 8:11 (22 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe improved methodology for collecting xylem sap and a novel GC-MS procedure for quantifying the presence of free and bound forms of abscisic acid (ABA). Although the abundance of these bound forms of ABA in tomato is found to be low, the technique will be of particular relevance for those species where bound forms are known to represent a much more significant fraction of the total xylem sap ABA content.
		Research In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation by genistein Sachiko Arase, Megumi Kasai, Akira Kanazawa Plant Methods 2012, 8:10 (19 March 2012) Abstract \| Provisional PDF \| PubMed
		Methodology A novel procedure for absolute real-time quantification of gene expression patterns Yingqing Lu, Lulu Xie, Jiani Chen Plant Methods 2012, 8:9 (9 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Standard methods for quantifying gene transcripts yield relative rather than absolute data (e.g. fold change compared to control treatments). The authors describe a real-time PCR method that enables absolute quantification of cDNAs. The objective is to allow the comparison of gene expression between different tissues, developmental stages, and even different experiments.
		Methodology Isolation of dimorphic chloroplasts from the single-cell C₄species Bienertia sinuspersici Shiu-Cheung Lung, Makoto Yanagisawa, Simon DX Chuong Plant Methods 2012, 8:8 (6 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary C4-type photosynthesis is of major scientific interest because of its greater efficiency compared to the more common C3-type. Bienertia sinuspersici belongs to a select group of just three terrestrial higher plant species known to perform C4 photosynthesis without the segregation of different parts of the pathway into different cell types. It achieves this feat by partitioning its photosynthetic functions between two distinct types of chloroplast that are located in separate cytoplasmic compartments of the same cell. The authors describe methodology for isolating the two types of chloroplast, providing a means to investigate the biochemistry and physiology that underlies this intriguing photosynthetic system.
		Software Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape Andrew P French, Michael H Wilson, Kim Kenobi, Daniela Dietrich, Ute Voss, Susana Ubeda-Tomas, Tony P Pridmore, Darren M Wells Plant Methods 2012, 8:7 (2 March 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Cell-o-tape is a software tool for the analysis of confocal laser scanning microscope images that facilitates the counting and measurement of individual cells along defined cell files. This provides a less subjective alternative to laborious manual quantification of the geometry of plant organs at the cellular scale. As an example, the authors demonstrate how the tool can automate the process of locating the position of the transition zone in an Arabidopsis root.
		Research A rice transient assay system identifies a novel domain in NRR required for interaction with NH1/OsNPR1 and inhibition of NH1-mediated transcriptional activation Mawsheng Chern, Wei Bai, Wing Hoi Sze-To, Patrick E Canlas, Laura E Bartley, Pamela C Ronald Plant Methods 2012, 8:6 (21 February 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors have developed an improved protoplast-based transient expression assay that provides an efficient way of studying transcription factors and their protein-protein interactions. They have gone on to use the system to identify a novel bipartite protein interaction domain that is conserved in higher plants and that is likely to be important in the regulation of innate immunity to pathogens.
		Methodology PhosphoRice: a meta-predictor of rice-specific phosphorylation sites Shufu Que, Kuan Li, Min Chen, Yongfei Wang, Qiaobin Yang, Wenfeng Zhang, Baoqian Zhang, Bangshu Xiong, Huaqin He Plant Methods 2012, 8:5 (3 February 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary PhosphoRice is a web-based tool specifically designed to predict phosphorylation sites in rice. It can be accessed at http://bioinformatics.fafu.edu.cn/PhosphoRice.
		Methodology A simple and efficient method for the long-term preservation of plant cell suspension cultures Anne-Marie Boisson, Elisabeth Gout, Richard Bligny, Corinne Rivasseau Plant Methods 2012, 8:4 (30 January 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe a very convenient way to preserve plant cell cultures that doesn't require any specialised equipment and avoids the use of freezing. A combination of low temperature (5oC) and a phosphate-free medium allows the cells to remain viable for 6 months and, importantly to resume active growth without a lag when they are returned to standard culture conditions.
		Methodology Hyperspectral imaging for small-scale analysis of symptoms caused by different sugar beet diseases Anne-Katrin Mahlein, Ulrike Steiner, Christian Hillnhütter, Heinz-Wilhelm Dehne, Erich-Christian Oerke Plant Methods 2012, 8:3 (24 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction Hongbo Liu, Xianghua Li, Jinghua Xiao, Shiping Wang Plant Methods 2012, 8:2 (15 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts Djédoux Angaman, Rocco Petrizzo, Francesc Hernández-Gras, Carmen Romero-Segura, Irene Pateraki, Montserrat Busquets, Albert Boronat Plant Methods 2012, 8:1 (13 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars Khirod K Sahoo, Amit K Tripathi, Ashwani Pareek, Sudhir K Sopory, Sneh L Singla-Pareek Plant Methods 2011, 7:49 (30 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology Comparative evaluation of extraction methods for apoplastic proteins from maize leaves Katja Witzel, Muhammad Shahzad, Andrea Matros, Hans-Peter Mock, Karl H Mühling Plant Methods 2011, 7:48 (22 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology Reverse protection assay: a tool to analyze transcriptional rates from individual promoters Yan O Zubo, Victor V Kusnetsov, Thomas Börner, Karsten Liere Plant Methods 2011, 7:47 (20 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea Scott R Clemow, Lindsey Clairmont, Lene H Madsen, Frédérique C Guinel Plant Methods 2011, 7:46 (15 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe a set of tools that should greatly facilitate investigations of both the mycorrhizal and rhizobial symbioses with pea. The tools include an improved transformation method and techniques for targeted inoculation of roots with the symbiont to enable the processes involved in establishing the symbiosis to be studied in more detail.
		Methodology An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants Bridget V Hogg, Joanna Kacprzyk, Elizabeth M Molony, Conor O'Reilly, Thomas F Gallagher, Patrick Gallois, Paul F McCabe Plant Methods 2011, 7:45 (13 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A very straightforward and generally applicable way of investigating apoptotic-like programmed cell death (AL-PCD) in plants is described. The method should be suitable for applications where high-throughput screening is needed - such as identifying mutants defective in genes involved in AL-PCD.
		Methodology A novel machine-vision-based facility for the automatic evaluation of yield-related traits in rice Lingfeng Duan, Wanneng Yang, Chenglong Huang, Qian Liu Plant Methods 2011, 7:44 (12 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe an automated facility that can enable the high-throughput evaluation of yield-related traits in rice. The facility could greatly facilitate the phenotyping process in breeding, genetics and functional genomics.
		Methodology High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes Seosamh Ó Lochlainn, Stephen Amoah, Neil S Graham, Khalid Alamer, Juan J Rios, Smita Kurup, Andrew Stoute, John P Hammond, Lars Østergaard, Graham J King, Phillip J White, Martin R Broadley Plant Methods 2011, 7:43 (8 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors demonstrate how high resolution melt analysis can be a useful tool to characterise allelic series of EMS-induced mutations, as generated for example in TILLING populations.
		Methodology A novel Gateway^®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens Sy Traore, Bingyu Zhao Plant Methods 2011, 7:42 (7 December 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary This new plasmid vector will make high-throughput functional analysis of plant genes more straightforward by allowing Gateway cloning directly into Agrobacterium tumefaciens.

	All
		Methodology A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction Jana Svacinova, Ondrej Novak, Lenka Plackova, Rene Lenobel, Josef Holik, Miroslav Strnad, Karel Dolezal Plant Methods 2012, 8:17 (17 May 2012) Abstract \| Provisional PDF \| Editor’s summary A microscale high-throughput method for the analysis of the cytokinin content of plant tissues.
		Methodology The Arabidopsis thaliana - Alternaria brassicicola pathosystem: a model interaction for investigating seed transmission of necrotrophic fungi. Stephanie Pochon, Emmanuel Terrasson, Thomas Guillemette, Beatrice Iacomi-Vasilescu, Sonia Georgeault, Marjorie Juchaux, Romain Berruyer, Isabelle Debeaujon, Philippe Simoneau, Claire Campion Plant Methods 2012, 8:16 (9 May 2012) Abstract \| Provisional PDF \| PubMed
		Methodology Protocol: optimised electrophysiological analysis of intact guard cells from Arabidopsis Zhong-Hua Chen, Cornelia Eisenach, Xin-Qin Xu, Adrian Hills, Michael R. Blatt Plant Methods 2012, 8:15 (6 May 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Guard cells are functionally important in their role regulating stomatal aperture and offer many advantages for electrophysiological analysis. The detailed protocols for voltage clamp analysis of Arabidopsis guard cells set out here allow measurements of much longer duration than was previously possible.
		Methodology Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies Andrea Pitzschke, Helene Persak Plant Methods 2012, 8:14 (4 May 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Transient expression is an extremely valuable component of the molecular biologist's toolbox. The authors describe a new version of this tool that promises to make the procedure more reliable - as well as being accessible to more laboratories.
		Methodology A novel fluorescent assay for sucrose transporters Peter J Gora, Anke Reinders, John M Ward Plant Methods 2012, 8:13 (4 April 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Budding yeast is an important model system for the heterologous expression of plant sucrose transporters. The authors have exploited the ability of type I sucrose uptake transporters to transport the fluorescent β-glucoside, esculin, to develop a rapid and sensitive assay system that should be a valuable tool for the functional analysis of members of this sucrose transporter family.
		Methodology Targeted parallel sequencing of large genetically-defined genomic regions for identifying mutations in Arabidopsis Kun-hsiang Liu, Matthew McCormack, Jen Sheen Plant Methods 2012, 8:12 (30 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Next generation sequencing (NGS) is becoming a powerful tool for identifying mutations at the molecular level. This paper describes an innovation that has the potential to make the NGS approach accessible to many more laboratories. In the targeted parallel sequencing (TPSeq) method only genetically defined genomic regions are sequenced, making the process less expensive and the downstream bioinformatics more straightforward.
		Methodology Xylem sap collection and extraction methodologies to determine in vivo concentrations of ABA and its bound forms by gas chromatography-mass spectrometry (GC-MS) Andrew G Netting, Julian C Theobald, Ian C Dodd Plant Methods 2012, 8:11 (22 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe improved methodology for collecting xylem sap and a novel GC-MS procedure for quantifying the presence of free and bound forms of abscisic acid (ABA). Although the abundance of these bound forms of ABA in tomato is found to be low, the technique will be of particular relevance for those species where bound forms are known to represent a much more significant fraction of the total xylem sap ABA content.
		Research In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation by genistein Sachiko Arase, Megumi Kasai, Akira Kanazawa Plant Methods 2012, 8:10 (19 March 2012) Abstract \| Provisional PDF \| PubMed
		Methodology A novel procedure for absolute real-time quantification of gene expression patterns Yingqing Lu, Lulu Xie, Jiani Chen Plant Methods 2012, 8:9 (9 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Standard methods for quantifying gene transcripts yield relative rather than absolute data (e.g. fold change compared to control treatments). The authors describe a real-time PCR method that enables absolute quantification of cDNAs. The objective is to allow the comparison of gene expression between different tissues, developmental stages, and even different experiments.
		Methodology Isolation of dimorphic chloroplasts from the single-cell C₄species Bienertia sinuspersici Shiu-Cheung Lung, Makoto Yanagisawa, Simon DX Chuong Plant Methods 2012, 8:8 (6 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary C4-type photosynthesis is of major scientific interest because of its greater efficiency compared to the more common C3-type. Bienertia sinuspersici belongs to a select group of just three terrestrial higher plant species known to perform C4 photosynthesis without the segregation of different parts of the pathway into different cell types. It achieves this feat by partitioning its photosynthetic functions between two distinct types of chloroplast that are located in separate cytoplasmic compartments of the same cell. The authors describe methodology for isolating the two types of chloroplast, providing a means to investigate the biochemistry and physiology that underlies this intriguing photosynthetic system.
		Software Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape Andrew P French, Michael H Wilson, Kim Kenobi, Daniela Dietrich, Ute Voss, Susana Ubeda-Tomas, Tony P Pridmore, Darren M Wells Plant Methods 2012, 8:7 (2 March 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Cell-o-tape is a software tool for the analysis of confocal laser scanning microscope images that facilitates the counting and measurement of individual cells along defined cell files. This provides a less subjective alternative to laborious manual quantification of the geometry of plant organs at the cellular scale. As an example, the authors demonstrate how the tool can automate the process of locating the position of the transition zone in an Arabidopsis root.
		Research A rice transient assay system identifies a novel domain in NRR required for interaction with NH1/OsNPR1 and inhibition of NH1-mediated transcriptional activation Mawsheng Chern, Wei Bai, Wing Hoi Sze-To, Patrick E Canlas, Laura E Bartley, Pamela C Ronald Plant Methods 2012, 8:6 (21 February 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors have developed an improved protoplast-based transient expression assay that provides an efficient way of studying transcription factors and their protein-protein interactions. They have gone on to use the system to identify a novel bipartite protein interaction domain that is conserved in higher plants and that is likely to be important in the regulation of innate immunity to pathogens.
		Methodology PhosphoRice: a meta-predictor of rice-specific phosphorylation sites Shufu Que, Kuan Li, Min Chen, Yongfei Wang, Qiaobin Yang, Wenfeng Zhang, Baoqian Zhang, Bangshu Xiong, Huaqin He Plant Methods 2012, 8:5 (3 February 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary PhosphoRice is a web-based tool specifically designed to predict phosphorylation sites in rice. It can be accessed at http://bioinformatics.fafu.edu.cn/PhosphoRice.
		Methodology A simple and efficient method for the long-term preservation of plant cell suspension cultures Anne-Marie Boisson, Elisabeth Gout, Richard Bligny, Corinne Rivasseau Plant Methods 2012, 8:4 (30 January 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe a very convenient way to preserve plant cell cultures that doesn't require any specialised equipment and avoids the use of freezing. A combination of low temperature (5oC) and a phosphate-free medium allows the cells to remain viable for 6 months and, importantly to resume active growth without a lag when they are returned to standard culture conditions.
		Methodology Hyperspectral imaging for small-scale analysis of symptoms caused by different sugar beet diseases Anne-Katrin Mahlein, Ulrike Steiner, Christian Hillnhütter, Heinz-Wilhelm Dehne, Erich-Christian Oerke Plant Methods 2012, 8:3 (24 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction Hongbo Liu, Xianghua Li, Jinghua Xiao, Shiping Wang Plant Methods 2012, 8:2 (15 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts Djédoux Angaman, Rocco Petrizzo, Francesc Hernández-Gras, Carmen Romero-Segura, Irene Pateraki, Montserrat Busquets, Albert Boronat Plant Methods 2012, 8:1 (13 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars Khirod K Sahoo, Amit K Tripathi, Ashwani Pareek, Sudhir K Sopory, Sneh L Singla-Pareek Plant Methods 2011, 7:49 (30 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology Comparative evaluation of extraction methods for apoplastic proteins from maize leaves Katja Witzel, Muhammad Shahzad, Andrea Matros, Hans-Peter Mock, Karl H Mühling Plant Methods 2011, 7:48 (22 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology Reverse protection assay: a tool to analyze transcriptional rates from individual promoters Yan O Zubo, Victor V Kusnetsov, Thomas Börner, Karsten Liere Plant Methods 2011, 7:47 (20 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea Scott R Clemow, Lindsey Clairmont, Lene H Madsen, Frédérique C Guinel Plant Methods 2011, 7:46 (15 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe a set of tools that should greatly facilitate investigations of both the mycorrhizal and rhizobial symbioses with pea. The tools include an improved transformation method and techniques for targeted inoculation of roots with the symbiont to enable the processes involved in establishing the symbiosis to be studied in more detail.
		Methodology An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants Bridget V Hogg, Joanna Kacprzyk, Elizabeth M Molony, Conor O'Reilly, Thomas F Gallagher, Patrick Gallois, Paul F McCabe Plant Methods 2011, 7:45 (13 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A very straightforward and generally applicable way of investigating apoptotic-like programmed cell death (AL-PCD) in plants is described. The method should be suitable for applications where high-throughput screening is needed - such as identifying mutants defective in genes involved in AL-PCD.
		Methodology A novel machine-vision-based facility for the automatic evaluation of yield-related traits in rice Lingfeng Duan, Wanneng Yang, Chenglong Huang, Qian Liu Plant Methods 2011, 7:44 (12 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors describe an automated facility that can enable the high-throughput evaluation of yield-related traits in rice. The facility could greatly facilitate the phenotyping process in breeding, genetics and functional genomics.
		Methodology High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes Seosamh Ó Lochlainn, Stephen Amoah, Neil S Graham, Khalid Alamer, Juan J Rios, Smita Kurup, Andrew Stoute, John P Hammond, Lars Østergaard, Graham J King, Phillip J White, Martin R Broadley Plant Methods 2011, 7:43 (8 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The authors demonstrate how high resolution melt analysis can be a useful tool to characterise allelic series of EMS-induced mutations, as generated for example in TILLING populations.
		Methodology A novel Gateway^®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens Sy Traore, Bingyu Zhao Plant Methods 2011, 7:42 (7 December 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary This new plasmid vector will make high-throughput functional analysis of plant genes more straightforward by allowing Gateway cloning directly into Agrobacterium tumefaciens.