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Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici
Shiu-Cheung Lung, Makoto Yanagisawa, Simon DX Chuong Plant Methods 2012, 8:8 (6 March 2012)
Abstract | Full text | PDF | PubMed
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Editor’s summary
C4-type photosynthesis is of major scientific interest because of its greater efficiency compared to the more common C3-type. Bienertia sinuspersici belongs to a select group of just three terrestrial higher plant species known to perform C4 photosynthesis without the segregation of different parts of the pathway into different cell types. It achieves this feat by partitioning its photosynthetic functions between two distinct types of chloroplast that are located in separate cytoplasmic compartments of the same cell. The authors describe methodology for isolating the two types of chloroplast, providing a means to investigate the biochemistry and physiology that underlies this intriguing photosynthetic system.
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