Open Access Open Badges Methodology

FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei

Filipe Borges, Rui Gardner, Telma Lopes, Joseph P Calarco, Leonor C Boavida, R Keith Slotkin, Robert A Martienssen and Jörg D Becker*

Plant Methods 2012, 8:44  doi:10.1186/1746-4811-8-44

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PEB buffer

Jorg Becker   (2013-08-08 12:53)  Instituto Gulbenkian de Ciencia email

Pollen Extraction Buffer (PEB) should be prepared with 0.1% H3BO3 and not 1% as indicated.
Alternatively SEB buffer can be used for microspore sorting, too.

Competing interests

No competing interests


Corrections for composition of buffer solutions

Jorg Becker   (2012-12-21 10:16)  Instituto Gulbenkian de Ci��ncia email

We have detected the following mistakes in the composition of two buffer solutions:

Galbraith buffer: It should read 0.1% (v/v) Triton-100 instead of 1% Triton-100

Sperm extraction buffer (SEB): It should read 0.83 mM MgSO4 instead of 5.83 mM MgSO4

We apologize to readers for these mistakes.

Competing interests

No competing interests


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