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Open Access Methodology

Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Zhong-Hua Chen12, Cornelia Eisenach1, Xin-Qin Xu1, Adrian Hills1 and Michael R Blatt1*

Author Affiliations

1 Laboratory of Plant Physiology and Biophysics, Institute of Molecular, Cell and Systems Biology, University of Glasgow, Glasgow, G12 8QQ, UK

2 School of Science and Health, University of Western Sydney, Richmond, NSW 2753, Australia

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Plant Methods 2012, 8:15  doi:10.1186/1746-4811-8-15

Published: 6 May 2012

Abstract

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.

Keywords:
Microelectrode; K+ channel (voltage-gated); Cl- channel, Voltage-gated; Membrane conductance; Mutant analysis; Arabidopsis