Fingerprinting antioxidative activities in plants

doi:10.1186/1746-4811-5-2

Plant Methods

Volume 5

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Fingerprinting antioxidative activities in plants

Livia Saleh and Christoph Plieth

Zentrum für Biochemie und Molekularbiologie, Universität Kiel, Am Botanischen Garten 9, 24118 Kiel, Germany

author email corresponding author email

Plant Methods 2009, 5:2doi:10.1186/1746-4811-5-2


Published:	26 January 2009

Additional files

Additional file 1:

The TAC Assay. The data provide information about optimal assay conditions for maximal light output. Further information is given for calibration and tuning the assay sensitivity. Fig. 1.1 The light emitting luminol reaction. Fig. 1.2 Enhanced versus not enhanced HRP-catalysed luminol reaction. Fig. 1.3 The pH-optimum of the HRP-catalysed luminol reaction. Fig. 1.4 The peroxide inactivation or 'suicide reaction'. Eq. 1 Definition Quenching. Fig. 1.5 Calibration of luminescence recovery times. Fig. 1.6 Tuning the TAC assay sensitivity. Fig. 1.7 Stability of the TAC assay mix

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The LUPO Assay. The data provide information about optimal assay conditions for maximal light output. Further information is provided about H₂O₂-sensitivity, calibration in terms of a purified peroxidase, and the heat sensitivity of Lepidium LUPOs. Fig. 2.1 The luminol converting peroxidase (LUPO) cycle. Fig. 2.2 Peroxidases from Lepidium sativum are not inactivated by H₂O₂. Fig. 2.3 The luminol reaction catalysed by purified lignin peroxidase. Fig. 2.4 Total light yield of the non-enhanced luminol reaction. Fig. 2.5 LUPOs from Lepidium are heat-sensitive.

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The SOSA Assay. The data provide information how to calibrate the SOSA assay in terms of a purified superoxide dismutase. Further information is given about different coelenterazine analogues, their performance as superoxide indicators, and their optimal concentration in the SOSA assay. Fig. 3.1 Superoxide generation and coelenterazine-mediated light emission. Tab. 1 Coelenterazine analogues tested for the SOSA assay. Fig. 3.2 The luminescence quenching correlates with SOSA. Fig. 3.3 The CTZ analogue is crucial for the SOSA assay performance. Fig. 3.4 CTZ concentration determines the duration of light output.

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The experimental design. The figures summarize the chronology of plant growth, treatment, and harvest and give a scheme how to process the biological material. Fig. 4.1 Experimental design. Fig. 4.2 Flow chart for processing plant material

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