Plant Methods

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Open Access

Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

Antoine LF Gady*, Freddy WK Hermans, Marion HBJ Van de Wal, Eibertus N van Loo, Richard GF Visser and Christian WB Bachem

Plant Methods 2009, 5:13 doi:10.1186/1746-4811-5-13

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BioMed Central: 3 citations

Methodology   Open Access

High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes

Seosamh Ó Lochlainn, Stephen Amoah, Neil S Graham, Khalid Alamer, Juan J Rios, Smita Kurup, Andrew Stoute, John P Hammond, Lars Østergaard, Graham J King, Phillip J White, Martin R Broadley Plant Methods 2011, 7:43 (8 December 2011)

The authors demonstrate how high resolution melt analysis can be a useful tool to characterise allelic series of EMS-induced mutations, as generated for example in TILLING populations.

Research article   Open Access

High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes

Ermelinda Botticella, Francesco Sestili, Antonio Hernandez-Lopez, Andrew Phillips, Domenico Lafiandra BMC Plant Biology 2011, 11:156 (10 November 2011)

Research article   Open Access Highly Accessed

A new mutant genetic resource for tomato crop improvement by TILLING technology

Silvia Minoia, Angelo Petrozza, Olimpia D'Onofrio, Florence Piron, Giuseppina Mosca, Giovanni Sozio, Francesco Cellini, Abdelhafid Bendahmane, Filomena Carriero BMC Research Notes 2010, 3:69 (12 March 2010)