3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei
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* Corresponding author: Valérie Gaudin Valerie.Gaudin@versailles.inra.fr
Plant Methods 2009, 5:11 doi:10.1186/1746-4811-5-11
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Review
Julia Grassl, Nicolas L Taylor, A Harvey Millar Plant Methods 2011, 7:21 (5 July 2011) MALDI-MSI is a powerful technique that provides high resolution information on the spatial arrangement of peptides and proteins in a tissue. This review highlights the advances made as well as the bottlnecks to further progress in developing the technique for plant applications. The authors also provide a 'beginner¿s guide' to MALDI-MSI in the form of a detailed experimental protocol.
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