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Technical error rates for metabolite profiling of Chlamydomonas reinhardtii cells. |
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| Homogenization |
Solvent system |
Identified metabolites |
All peaks |
|
|
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| glass beads |
MCW |
30% |
39% |
| glass beads |
WiPM |
26% |
34% |
| steel ball |
MCW study1 |
16% |
22% |
| steel ball |
MCW study2 |
18% |
23% |
| steel ball |
WiPM study1 |
21% |
30% |
| steel ball |
WiPM study2 |
25% |
38% |
| steel ball |
MeOH |
30% |
40% |
| steel ball |
WiPA |
28% |
38% |
| steel ball |
MCW (10/3/1) |
30% |
37% |
|
The median precision (as relative coefficient of variance) is given for the 80 compounds identified by BinBase, resp. over all 334 peaks that were annotated metabolic signals by BinBase. Study 1 comprised the comparison of grinding methods, study 2 compared different solvents. Abbreviation of solvents as given in figures 3–5. | |||
Lee and Fiehn Plant Methods 2008 4:7 doi:10.1186/1746-4811-4-7 |
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