Plant Methods
Volume 4

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Costa A
Leonhardt N
Siegel RS
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Costa A
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Methodology

Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool

Yingzhen Yang¹ , Alex Costa² , Nathalie Leonhardt³ , Robert S Siegel¹ and Julian I Schroeder¹

¹Cell and Developmental Biology Section, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0116, USA

²Department of Biology, University of Padua, Via U. Bassi 58/B, I-35131, Padova, Italy

³CEA Cadarache, DSV, UMR 6191 CEA-CNRS, DEVM, LEMS and LEMP, St Paul les Durance Cedex, France

author email corresponding author email

Plant Methods 2008, 4:6doi:10.1186/1746-4811-4-6


Published:	19 February 2008

Additional files

Additional file 1:

Experiment I-Guard cells without ABA (JS33.xls). Actinomycin and Cordycepin were added during protoplast isolation.

Format: XLS Size: 4.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Experiment I-Guard cells treated with 100 μM ABA (JS34.xls). Actinomycin and Cordycepin were added during protoplast isolation.

Format: XLS Size: 4.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Experiment I-Mesophyll cells without ABA (JS35.xls). Actinomycin and Cordycepin were added during protoplast isolation.

Format: XLS Size: 4.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 4:

Experiment I-Mesophyll cells treated with 100 μM ABA (JS36.xls). Actinomycin and Cordycepin were added during protoplast isolation.

Format: XLS Size: 4.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 5:

Experiment II-Guard cells without ABA (JS85.xls).

Format: XLS Size: 6.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 6:

Experiment II-Guard cells treated with 100 μM ABA (JS86.xls)

Format: XLS Size: 6.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 7:

Experiment II-Mesophyll cells without ABA (JS87.xls).

Format: XLS Size: 6.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 8:

Experiment II-Mesophyll cells treated with 100 μM ABA (JS88.xls).

Format: XLS Size: 6.6MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 9:

Guard cell promoter candidate gene expression in GC and MC.

Format: DOC Size: 76KB Download file

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Additional file 10:

Representative movie 1 of spontaneous calcium transients occurring in guard cells of intact pGC1::YC3.60 transgenic plants. The time compression factor is 30 times.

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Additional file 11:

Representative movie 2 of spontaneous calcium transients occurring in guard cells of intact pGC1::YC3.60 transgenic plants. The time compression factor is 30 times.

Format: AVI Size: 5.3MB Download file

Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more

Additional file 12:

Multiple (T/A)AAAG elements are present in all the examined promoters.

Format: DOC Size: 59KB Download file

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