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Oligonucleotide combinations used for amplification of NbPDS VIGS inserts |
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| Construct name |
Primer combination |
|
|
|
| NbPDS (2046 bp) |
NbPDSfor1 × NbPDSrev1 |
| NbPDS (1661 bp) |
NbPDSfor2 × NbPDSrev1 |
| NbPDS (1304 bp) |
NbPDSfor3 × NbPDSrev1 |
| NbPDS (959 bp) |
NbPDSfor4 × NbPDSrev1 |
| NbPDS (610 bp) |
NbPDSfor5 × NbPDSrev1 |
| NbPDS (257 bp) |
NbPDSfor6 × NbPDSrev1 |
| NbPDS (345 bp) |
NbPDSfor3 × NbPDSrev2 |
| NbPDS (192 bp) |
NbPDSfor7 × NbPDSrev3 |
| NbPDS (103 bp) |
NbPDSfor7 × NbPDSrev4 |
| NbPDS (54 bp) |
NbPDSfor8 × NbPDSrev5 |
| NbPDS (385 bp) |
NbPDSfor1 × NbPDSrev6 |
| NbPDS (357 bp) |
NbPDSfor2 × NbPDSrev7 |
| NbPDS (349 bp) |
NbPDSfor4 × NbPDSrev8 |
| NbPDS (353 bp) |
NbPDSfor5 × NbPDSrev9 |
| NbPDS (2046 bp + polyA) |
NbPDSfor1 × NbPDSrev6 |
| NbPDS (1661 bp + polyA) |
NbPDSfor2 × NbPDSrev6 |
| NbPDS (1304 bp + polyA) |
NbPDSfor3 × NbPDSrev6 |
| NbPDS (959 bp + polyA) |
NbPDSfor4 × NbPDSrev6 |
| NbPDS (610 bp + polyA) |
NbPDSfor5 × NbPDSrev6 |
| NbPDS (257 bp + polyA) |
NbPDSfor6 × NbPDSrev6 |
| NbPDS (345 bp + polyA) |
NbPDSfor7 × NbPDSrev7 |
| NbPDS (345 bp + polyG) |
NbPDSfor7 × NbPDSrev8 |
Liu and Page Plant Methods 2008 4:5 doi:10.1186/1746-4811-4-5 |
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