Figure 1.

Schematic from PCR gene cloning to Agrobacterium expression clones using the Gateway^® cloning technology. Col E1 ori: origin of replication for E. coli. pVS1 ori: origin of replication for Agrobacteria. Kan^r: kanamycin resistance gene. Cm^r: chloramphenicol resistance gene. RB: T-DNA right border. LB: T-DNA left border. attL, attR, attB: recombination sites. ccdB: E. coli ccdB gene (note: the ccdB protein interferes with E. coli DNA gyrase and inhibits growth of most E. coli strains, e.g., DH5α™).