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Tests of functionality of the newly generated constructs. |
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| REal – derived pENTR constructs |
SSR – derived pDEST construct |
Expression in N. benthamiana |
Expression in Arabidopsis |
|
|
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| pE2n-H2A |
pH2n-H2A |
Yes |
Yes |
| pE2c-H2A |
pH2c-H2A |
Yes |
Yes |
| pE2n-H2B |
pH2n-H2B |
Yes |
Yes |
| pE2c-H2B |
pH2c-H2B |
Yes |
Yes |
| pE1c-H2B |
pB1c-H2B |
NT |
Yes |
| pE2n-UBI |
pH-2n-UBI |
Yes |
Yes |
| pE6n-UBI |
pB6n-UBI |
NT |
Yes |
| pE5n-UBI |
pH5n-UBI |
NT |
Yes |
| pE1n-UBI |
pB1n-UBI |
NT |
Yes |
| pE3n-DET1 |
pK3n-DET1 |
Yes |
NT |
| pE6n-DET1 |
pB6n-DET1 |
NT |
No |
| pE6n-DET1 |
pM6n-DET1 |
NT |
Yes |
| pE1n-DET1 |
pB1n-DET1 |
Yes |
No |
| pE2n-DDB1 |
pH2n-DDB1 |
Yes |
NT |
| pE3n-DDB1 |
pK3n-DDB1 |
Yes |
NT |
| pE2c-DDB1 |
pH2c-DDB1 |
Yes |
NT |
| pE3c-DDB1 |
pK3c-DDB1 |
Yes |
NT |
|
Table shows constructs made and their functionality by expression analysis in N. benthamiana and in transgenic Arabidopsis plants. Protein extracts from transiently transformed tobacco leaves or from Arabidopsis homozygous lines were analysed by western blotting using the appropriate antibodies. Yes, expressed. No, not expressed. NT, not tested. pH, pH2GW7. pK, pK2GW7. pB, pB2GW7. pM, pMDC7. | |||
Dubin et al. Plant Methods 2008 4:3 doi:10.1186/1746-4811-4-3 |
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