Figure 3.

Recombination event in plant binary Destination vectors. Schematic drawing of the recombination between the newly generated pENT-TAG-GENE vectors and a Destination vector. The recombination events between the attL and attR sites that generate attB sites occur outside the ORF encoding the tagged protein of interest so no additional sequences are added. A variety of assembled plant binary vectors can be generated with different promoters and resistance markers.