Figure 1.

PCR products using selected primers of precipitated DNA from SSR-enriched libraries. A 1 kb molecular size standard was loaded on each side and in the middle of the gel. A total of 3 μl of PCR products were loaded in each lane to evaluate the primer combinations (all four primers vs. three primer combinations of MseI -A, -T, -C, or -G) showing the least duplication (bands) of PCR products. The primer combinations without MseI -C and -G were selected to generate the SSR-enriched libraries.