|
Organ-enhanced TF genes |
|||||||||||
| A Root-enhanced TF genes identified by real-time RT-PCR |
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|
|
|||||||||||
| Real-time RT-PCR Expression Ratio |
Affymetrix Expression Ratio |
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|
|
|||||||||||
| Accession Number |
R/L |
R/S |
R/F |
R/P |
R/N |
R/L |
R/S |
R/F |
R/P |
R/N |
Affymetrix Chip ID |
|
|
|||||||||||
| AC140721_12.1 |
121.5 |
320.1 |
1623.4 |
2676.5 |
64.4 |
161.2b |
144.5b |
219.2b |
158.1b |
99.9 |
Mtr.50075.1.S1_s_at |
| AC135101_25.1 |
312.2 |
438.0 |
291.6 |
787.0 |
61.3 |
n |
n |
n |
n |
n |
n |
| AC140721_14.1 |
1204.9 |
751.2 |
956.6 |
6296.6 |
32.6 |
n |
n |
n |
n |
n |
n |
| AC140031_3.1 |
275.4 |
1157.3 |
923.4 |
258916.9a |
31.1 |
0.4 |
0.7 |
0.8 |
0.9 |
2.2 |
Mtr.47227.1.S1_s_at |
| AC140721_13.1 |
39.9a |
179.8 |
614.8 |
935.1a |
26.5 |
35.9 |
48.1b |
41.2 |
44.2b |
39.5 |
Mtr.50074.1.S1_at |
| AC140031_7.1 |
360.2a |
830.6 |
1542a |
6868.8 |
19.7 |
0.7b |
1b |
1.1b |
1b |
0.9b |
Mtr.47229.1.S1_at |
| AC146574_6.1 |
1095.7 |
11.9a |
48.8 |
51.8 |
88.8 |
1.2b |
1.1b |
1.3b |
1.1b |
1.2b |
Mtr.40781.1.S1_s_at |
| AC125478_13.7 |
1117.4 |
7008.0 |
3264.9a |
7256.6 |
11.6 |
211.5b |
248.8b |
296.4b |
228.7b |
45.3 |
Mtr.15416.1.S1_at |
| AC125478_7.2 |
1052.3 |
503.3 |
938.4a |
2754.5 |
11.2 |
n |
n |
n |
n |
n |
n |
| AC122726_21.111 |
50.0 |
48.0 |
20943.9 |
2658.6 |
10.4 |
74.3b |
76.4b |
72.8b |
82.8b |
26.6 |
Mtr.15568.1.S1_s_at |
|
|
|||||||||||
| B Nodule-enhanced TF genes identified by real-time RT-PCR |
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|
|
|||||||||||
| Real-time RT-PCR Expression Ratio |
Affymetrix Expression Ratio |
||||||||||
|
|
|||||||||||
| Accession Number |
N/L |
N/S |
N/F |
N/P |
N/R |
N/L |
N/S |
N/F |
N/P |
N/R |
Affymetrix Chip ID |
|
|
|||||||||||
| AC148816_3.2 |
9966.7 |
1826.2 |
6262.9 |
21709.1 |
662.7 |
434.6b |
516.8b |
455b |
521.7b |
551.4b |
Mtr.14503.1.S1_at |
| AC147774_3.2 |
55.2a |
466.7 |
726.3 |
881.2 |
498.5 |
17.7b |
16b |
17.8b |
19.1b |
16.1b |
Mtr.19554.1.S1_at |
| AC138056_33.241 |
43.9 |
93.2 |
18.3 |
17.3 |
13.1 |
0.9b |
0.8b |
0.8b |
1.1b |
1.1b |
Mtr.17993.1.S1_at |
| AC124214_39.2 |
121.8 |
92.1 |
44.8 |
64.9 |
10.5 |
n |
n |
n |
n |
n |
n |
|
|
|||||||||||
| C Pod-enhanced TF genes identified by real-time RT-PCR |
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|
|
|||||||||||
| Real-time RT-PCR Expression Ratio |
Affymetrix Expression Ratio |
||||||||||
|
|
|||||||||||
| Accession Number |
P/L |
P/S |
P/F |
P/N |
P/R |
P/L |
P/S |
P/F |
P/N |
P/R |
Affymetrix Chip ID |
|
|
|||||||||||
| AC143340_4.7 |
154.1 |
44.8 |
173.3 |
180.3a |
407.3 |
1.6b |
1.8b |
1.7b |
1.7b |
1.7b |
Mtr.17931.1.S1_at |
|
|
|||||||||||
| D Flower-enhanced TF genes identified by real-time RT-PCR |
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|
|
|||||||||||
| Real-time RT-PCR Expression Ratio |
Affymetrix Expression Ratio |
||||||||||
|
|
|||||||||||
| Accession Number |
F/L |
F/S |
F/P |
F/N |
F/R |
F/L |
F/S |
F/P |
F/N |
F/R |
Affymetrix Chip ID |
|
|
|||||||||||
| AC129092_13.1 |
40.3 |
383.3 |
65.1 |
1683.9 |
318.1 |
24.2 |
47.1b |
38.2 |
45.4b |
55.1b |
Mtr.16432.1.S1_at |
| AC148485_10.1 |
476.2 |
203.7 |
27.2 |
174.6 |
44.5 |
36.5b |
32.9b |
22.0 |
34.7b |
29.7b |
Mtr.20392.1.S1_at |
| AC140915_20.1 |
169.5 |
101.1 |
18.6 |
481.0 |
178.7 |
1.4b |
1.4b |
1.3b |
1.3b |
1.6b |
Mtr.51688.1.S1_at |
| AC141107_50.2 |
38.2 |
33.0 |
17.9 |
114.0 |
97.4 |
n |
n |
n |
n |
n |
n |
| AC144731_15.21 |
64.4 |
44.3 |
33.6 |
61.5 |
16.3 |
0.6b |
0.6b |
0.8b |
0.8b |
0.8b |
Mtr.19093.1.S1_at |
| AC150978_12.1 |
235.3 |
198.5 |
48.4 |
14.0 |
16.9 |
n |
n |
n |
n |
n |
n |
| AC141107.5.61 |
261.1 |
127.8 |
13.3 |
96.4 |
71.5 |
0.9b |
0.9b |
0.8b |
0.1b |
1.1b |
Mtr.51651.1.S1_at |
| AC157472_19.1 |
60.2a |
92.1 |
13.2 |
61.5a |
113.6 |
n |
n |
n |
n |
n |
n |
| AC148527_19.141 |
188.4 |
42.1 |
17.3 |
26.5 |
13.0 |
n |
n |
n |
n |
n |
n |
| AC144726_6.1 |
254.7 |
55.2 |
10.9 |
112.7a |
754.7 |
59.2 |
73.7b |
7.6 |
89.2 |
97.9b |
Mtr.19024.1.S1_at |
| AC157488_16.1 |
7053.2a |
141.1 |
18.7 |
81.8 |
10.7 |
n |
n |
n |
n |
n |
n |
| AC123899_15.181 |
10.2 |
92.2 |
19.1 |
62.8 |
24.9 |
0.9b |
0.5b |
0.8b |
0.1b |
0.8b |
Mtr.52015.1.S1_at |
|
A TF gene was considered organ-enhanced if transcript levels for that gene were more than 10-fold higher in one organ than in any other organ. Transcript ratios were calculated using the mean of three biological replicates for each organ. Data from qRT-PCR are compared with data for the same RNA samples obtained from Affymetrix Gene chips [38]. Affymetrix data were normalized using the Robust Multiarray Average (RMA) method, as described by [38], prior to calculation of ratios. Data in bold represent the lowest transcript ratio of the corresponding gene across all organs. n = not present on Affymetrix chip; a = Ct > 40 in two or three biological replicates of denominator organ; b = transcript called 'absent' by Affymetrix software in two or three biological replicates of denominator organ. L = leaf; S = stem; P = pod; F = flower; R = root; N = nodule. | |||||||||||
Kakar et al. Plant Methods 2008 4:18 doi:10.1186/1746-4811-4-18 |
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