A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in Medicago truncatula

Additional file 1:

Complete list of TF genes, primer sequences and corresponding PCR efficiencies. The TF primer platform was established based on the data for the gene models according to SA, specific amplification; NA, no amplification; NS, non-specific amplification. RT-PCR products of primer sequences indicated in bold were sequenced.

Format: XLS Size: 305KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Complete list of TF genes, gene families and experimental data. Shown are the Medicago gene Accession Numbers (TIGR) in ascending order, the TC number if available, as well as the transcription factor family and the subfamily names. The next columns show experimental results for the real-time RT-PCR reactions performed on six different organs of Medicago in three indipendent biological replicates, as explained in the manuscript. CT = not normalized CT value, ΔCT = CT value normalized against the geometric mean of 4 house keeping genes; ΔΔCT = power(PCReff;-ΔCT); log2 ΔΔCT = logarithmus of ΔCT.

Format: XLS Size: 2.1MB Download file

This file can be viewed with: Microsoft Excel Viewer