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A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress

Silvia Forcat1, Mark H Bennett1, John W Mansfield1 and Murray R Grant2*

Author Affiliations

1 Biology Division, Imperial College London, Exhibition Road, London SW7 2AZ, UK

2 School of Biosciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK

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Plant Methods 2008, 4:16  doi:10.1186/1746-4811-4-16

Published: 30 June 2008


We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.