|
Excitation and emission wavelengths (in nm) used. |
||||
| excitation maximum |
laser line used |
emission maximum |
emission collected |
|
|
|
||||
| SYTOX blue |
444 |
458 |
480 |
475 to 500 |
| SYTOX green |
504 |
488 |
523 |
510 to 560 |
| SYTOX orange |
547 |
568 |
570 |
580 to 610 |
| GFP |
457 |
488 |
504 |
510 to 545 |
| Propidium iodide |
536 |
488 |
617 |
610 to 650 |
| Fluorescein diacetate |
494 |
488 |
518 |
505 to 525 |
|
The first and third columns show excitation and emission maxima of the fluorescent dyes and proteins used (as indicated by the suppliers). The second and fourth columns show the excitation and emission wavelengths used in our experiments. | ||||
Truernit and Haseloff Plant Methods 2008 4:15 doi:10.1186/1746-4811-4-15 |
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