Table 1

Selected reference genes for rice and corresponding primer pair information.
Locus identifier
 Gene name
 Primer sequence F/R [5'-3']
 Amplicon length [bp]
 Amplicon Tm [°C]
 PCR efficiency
Os03g50890
 Actin
 CTCCCCCATGCTATCCTTCG
TGAATGAGTAACCACGCTCCG[S]
 91
 81
 1.9
Os05g36290*
 Actin1
 ATCCTTGTATGCTAGCGGTCGA
ATCCAACCGGAGGATAGCATG[D]
 118
 80
 1.9
Os01g59150*
 β-Tubulin
 GGAGTCACATGCTGCCTAAGGTT
TCACTGCCAGCTTACGGAGG[S]
 64
 80
 1.96
Os06g11070
 Expressed protein
 AGGCTGGTCGAGGAGTCCAT
TTCTCCTCCCTAGCGAACACCT[D]
 101
 85
 1.83
Os03g55270
 TIP41-like
 GTTTGGATGAACCCCGCAA
GGCAACAAGGTCAATCCGATC[S]
 62
 77.5
 1.81
Os08g19610
 Cyclophilin
 CCACCATCACAGATCGGATCTT
GCGGTCAGAGCGAAAGTAGCTA[S]
 65
 84.2
 1.73
Os03g08020*
 Elongation factor 1α
 GTCATTGGCCACGTCGACTC
TGTTCATCTCAGCGGCTTCC[S]
 118
 83.5
 1.85

* Primer pair recognizes all splicing variants. Location of forward and reverse primer in the same exon [S] or in different exons [D].

Caldana et al. Plant Methods 2007 3:7   doi:10.1186/1746-4811-3-7