Figure 3.

Linearity and sensitivity of qRT-PCR. cDNA derived from root or shoot RNA was mixed in different ratios (as indicated; total amount of cDNA was 1 ng) and used as template to test transcript abundance of three selected genes (Os03g55610, Os08g38220, and Os12g38200) via qRT-PCR. A linear relationship between root (or shoot) cDNA and expression level of the various genes was observed. Symbols in both panels represent the mean ± SD (n = 3).