A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors

doi:10.1186/1746-4811-3-7

Plant Methods

Volume 3

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Caldana C
Scheible WR
Mueller-Roeber B
Ruzicic S

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Methodology

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors

Camila Caldana¹ , Wolf-Rüdiger Scheible¹ , Bernd Mueller-Roeber¹^,2 and Slobodan Ruzicic¹

¹Max-Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany

²University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Straße 24-25, Haus 20, 14476 Potsdam-Golm, Germany

author email corresponding author email

Plant Methods 2007, 3:7doi:10.1186/1746-4811-3-7


Published:	8 June 2007

Additional files

Additional file 1:

Complete list of TF genes, primer data and corresponding PCR efficiencies. The TF primer platform was established based on the data for the gene models according to the Version 2.0 of TIGR Rice genome annotation. The corresponding new identifiers (Version 5.0) are also presented. The ranking of primer pairs taking into account experimental data (Cat column) was done as follows: 1, specific amplification; 2, no amplification; 3, non-specific amplification. 'Primer Location' indicates the sites within genes selected for primer design: J, at least one of the primers extends over an exon-exon junction; D, the primers are located in different exons; S, both primers are located within same exon.

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Additional file 2:

cDNA samples used for the validation of the reference genes.

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