Figure 2.

The sensitivity of the stem-loop RT-PCR assay. A. Gel blot analyses of miR156, miR159 and miR167 expression. AS, antisense probe; S, sense probe. B. Stem-loop RT-PCR analyses of miR156, miR159 and miR167 expression. The amounts of RNA used for reverse transcription reactions are indicated on the top. PCR cycle numbers are indicated on the left.