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Development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a BAC minimum tile path

Kay L Shopinski2,3* email, Muhammad J Iqbal1* email, Jeffry L Shultz2,4 email, Dheepakkumaran Jayaraman2 email and David A Lightfoot2 email

Institute for Sustainable and Renewable Resources (ISRR), Institute for Advanced Learning and Research (IALR), Danville, VA 24540, USA

Department of Plant, Soil and Agriculture Systems, Room 176, Agriculture Building, MC 4415, Southern Illinois University, Carbondale, IL 62901, USA

Dept of Plant Molecular Biology, United States Department of Agriculture, Peoria, IL, USA

Dept of Soybean Genetics, United States Department of Agriculture, Stoneville, MS 38776, USA

author email corresponding author email* Contributed equally

Plant Methods 2006, 2:20doi:10.1186/1746-4811-2-20

Published: 8 December 2006

Additional files

Additional file 1:

Table of BACs identified in pool hybridizations of ESTs from the Gm-r1021 soybean cDNA library. The hybridized BACs are listed by common probe and Contig No. in ascending, numerical order.

Format: DOC Size: 348KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 2:

Table of BAC hybridizations with ESTs from the FiS soybean cDNA library. The hybridized BACs are listed by common probe and Contig No. in ascending, numerical order

Format: DOC Size: 408KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 3:

Table of ESTs found on major linkages groups of soybean in build 4. BACs that hybridized to ESTs sorted by linkage group to identify ESTs found on the different MLGs.

Format: DOC Size: 286KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 4:

Locations inferred in the build 3 version of the physical map of BAC clones hybridized to subtracted ESTs showing contig number and linkage group if determined. BACs that hybridized to ESTs in build 3 sorted by linkage group to identify ESTs found on the different MLGs.

Format: DOC Size: 494KB Download file

This file can be viewed with: Microsoft Word Viewer


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