Methodology

A petal breakstrength meter for Arabidopsis abscission studies

Kevin A Lease , Sung Ki Cho and John C Walker

Division of Biological Sciences, 303 Life Sciences Center, University of Missouri, Columbia, MO 65211, USA

author email corresponding author email

Plant Methods 2006, 2:2doi:10.1186/1746-4811-2-2

Published:	16 February 2006

Abstract

Background

Abscission is the regulated dropping of plant organs, such as leaves or flower petals. This process involves a break down of the cell wall between layers of cells in the abscission zone, causing the organ to become detached. The model plant Arabidopsis thaliana undergoes floral organ abscission. Various experimental methods have been used to study Arabidopsis floral organ abscission, including measuring the petal breakstrength, or the amount of force required to pull a petal from the receptacle. Petal breakstrength provides a quantitative insight into the physical integrity of the petal abscission zone.

Results

We developed a petal breakstrength meter that allows rapid data acquisition on a personal computer. We present the design of the device and show its utility in measuring Arabidopsis petal breakstrength for abscission studies.

Conclusion

This petal breakstrength meter should enable researchers to perform the petal breakstrength assay as a routine part of the characterization of environmental and genetic factors affecting abscission.