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Examples of plant protein-protein interactions studied via FRET or BiFC. |
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| Protein-protein interaction |
Applied technique |
Cell type |
Gene transfer method |
reference |
|
|
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| Phytochrome B-Cryptochrome 2 |
FRET (channel and DFRAP) |
Tobacco protoplasts |
Protoplast transfection |
[24] |
| SERK-1 (homodimerization) |
FRET (FSPIM) |
Cowpea mesophyll protoplasts |
Protoplast transfection |
[25] |
| Floral binding protein 2 (homodimerization) Floral binding protein 11 |
FRET (FSPIM and FLIM) |
Petunia leaf protoplasts |
Protoplast transfection |
[26] |
| TGA5 (homodimerization) |
FRET (channel) |
Tobacco leaf cells |
Agroinfiltration |
[27] |
| SERK1-KAPP |
FRET (FSPIM) |
Cowpea mesophyll protoplasts |
Protoplast transfection |
[28] |
| Opaque2-CGN5/ADA2 |
FRET (DFRAP and FLIM) |
Cowpea mesophyll protoplasts |
Protoplast transfection |
[29] |
| Lipidated YFP and CFP variants |
FRET (FSPIM and FLIM) |
Cowpea protoplasts |
Protolplast transfection |
[30] |
| AtMinD1 (homodimerization) |
FRET (channel and DFRAP) |
Tobacco leaf epidermal cells |
Particle bombardment |
[31] |
| MLO-calmodulin |
FRET (DFRAP and FLIM) |
Barley leaf epidermal cells |
Particle bombardment |
[32] |
| MLO (homodimerization) |
FRET (DFRAP) |
Barley leaf epidermal cells |
Particle bombardment |
[33] |
| Vacuolar ATPase subunits |
FRET (channel) |
Arabidopsis leaf mesophyll protoplasts |
Protoplast transfection |
[34] |
| MADS box proteins |
FRET (FLIM) |
Cowpea and Petunia leaf protoplasts |
Protoplast transfection |
[35] |
| SAG101-EDS1 |
FRET (DFRAP) |
Arabidopsis leaf epidermal cells |
Particle bombardment |
[36] |
| AtMinE1-AtMinD1, AtFtsZ1-1-AtFtsZ2-1, AtFtsZ2-1-ARC6 |
FRET (method unknown) |
Tobacco leaf epidermal cells |
Particle bombardment |
[37] |
| bZIP63 (homodimerization) |
BiFC |
Tobacco leaf epidermal cells |
Agro-infiltration |
[68] |
| LSD1 (homodimerization) |
BiFC |
Arabidopsis leaf epidermal cells |
Agro-infiltration |
[68] |
| 14-3-3 (homodimerization) |
BiFC |
Arabidopsis cell culture protoplasts and tobacco leaf epidermal cells |
Protoplast transfection and Agro-infiltration |
[68] |
| PFTα-PFTβ |
BiFC |
Arabidopsis leaf epidermal cells |
Agro-infiltration |
[69] |
| FIE-MEA |
BiFC |
Tobacco and Arabidopsis leaf epidermal cells |
Agro-infiltration |
[69] |
| VIP1-VirE2, VIP1-VirF |
BiFC |
Tobacco and onion leaf epidermal cells |
Particle bombardment |
[70] |
| SAD-GAMYB |
BiFC |
Onion leaf epidermal cells |
Particle bombardment |
[71] |
| OFP1 (homodimerization), BLH1 (homodimerization), AtOFP1-AtOFP1 |
BiFC |
Tobacco leaf cells |
Agroinfiltration |
[72] |
| VirE2-VirE3 |
BiFC |
Tobacco and onion leaf epidermal cells |
Particle bombardment |
[73] |
| VIP1-VirE2 |
BiFC |
Tobacco leaf epidermal cells |
Particle bombardment |
[74] |
| VIP1-H2A |
BiFC |
Tobacco leaf epidermal cells |
Particle bombardment |
[75] |
| EID1-ASK1 |
BiFC |
Mustard seedlings and parsley protoplasts |
Particle bombardment and protoplast transfection |
[76] |
| FD-FT |
BiFC |
Tobacco leaf epidermal cells |
Agro-infiltration |
[77] |
| AtMinE1-AtMinD1, AtFtsZ1-1-AtFtsZ2-1, AtFtsZ2-1-ARC6 |
BiFC |
Tobacco leaf epidermal cells |
Particle bombardment |
[37] |
| OsOBF1 (homodimerization), OsOBF1-LIP19 |
BiFC |
Onion bulb epidermal cells |
Particle bombardment |
[78] |
| ATH1-STM, BLH3-STM, BLH9-STM |
BiFC |
Leek epidermal cells |
Particle bombardment |
[79] |
| p6 and TGBp2 topology |
BiFC |
Tobacco leaf epidermal cells |
Particle bombardment and Agro-infiltration |
[80] |
Bhat et al. Plant Methods 2006 2:12 doi:10.1186/1746-4811-2-12 |
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