Figure 1.

Schematic representation of the basic applications of homologous recombination cloning in E. coli for genetic engineering. Homologous recombination cloning in E. coli can be used for gene replacement (A), insertion (B) or sub-cloning of target sequences into alternative plasmid vectors. The recombination is mediated by linear DNA fragments (usually generated by PCR), including target site-specific homology arms and a counter selectable antibiotic resistance gene marker.