Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli

Laura C Roden¹^,3 , Berthold Göttgens² and Effie S Mutasa-Göttgens¹

¹Broom's Barn Research Station, Higham, Bury St Edmunds, Suffolk IP28 6NP, UK

²Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 2XY, UK

³Dept. Mol. & Cell Biol., UCT, Private Bag Rondebosch, 7701, Cape Town, South Africa

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Plant Methods 2005, 1:6doi:10.1186/1746-4811-1-6


Published:	29 September 2005

Additional File 1:

PCR Primer Sequences. Details of the primers used isolate and manipulate the AtSTM gene locus by homologous recombination in E. coli EL250

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