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Summary of Agrobacterium strains and vectors used to investigate wheat transformation. |
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| Agrobacterium strain (binary vector) |
Chromosomal background |
Ti plasmid |
Opine classification |
Additional vir genes on binary or helper plasmids |
Binary type |
Selectable and scorable marker on T-DNA. (Promoter shown in parentheses) |
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| ABI (pMON18365) [17, 18] |
C58 |
Disarmed pTiC58 |
Nopaline |
pMON18365, none reported |
normal-binary |
nptII (E35S) GUS (E35S) |
| C58C1 (pPTN155) [15] |
C58 |
Cured/disarmed? |
Nopaline |
pPTN155, none reported |
normal-binary |
nptII (35S) GUS (E35S) |
| AGL1 (pAL154/156) [21] |
C58, RecA |
pTiBo542 ΔT-DNA |
Succinamopine |
pAL154, 15.2 Kb fragment from pTiBo542 [47], pAL156, none |
super-binary |
bar (Ubi1) GUS (Ubi1) |
| AGL0 (pBGX1 and pTO134) [19] |
C58 |
pTiBo542 ΔT-DNA |
Succinamopine |
pBGX1, none reported pTO134, none reported |
normal-binary |
hpt (35S) gfp (Ubi1) bar (35S) sgfpS65T (35S) |
| AGL1 (pDM805) |
C58, RecA |
pTiBo542 ΔT |
Succinamopine |
pDM805, none reported |
normal-binary |
bar (Ubi1) GUS (Act1) |
| LBA4404 (pTOK233) [45] |
Ach5 |
DNA Disarmed pAL4404 |
Octopine |
pTOK233, extra copy of virB, virC and virG from pTiBo542 47, [48] |
super-binary |
hpt (35S) GUS (35S) |
| LBA4404 (pHK21) [20] |
RecA Ach5 |
Disarmed pAL4404 |
Octopine |
pHK21, extra copy of virB, virC and virG from pTiBo542 [47] |
super-binary |
bar (Ubi1) GUS (Ubi1) |
| AGL1 (pUNN-2) [46] |
C58, RecA |
pTiBo542 ΔT-DNA |
Succinamopine |
pUNN-2, none reported |
normal-binary |
nptII (Ubi1)) |
| ABI (pMON30139 and others) [16] |
C58 |
Disarmed pTiC58 |
Nopaline |
pMON30139, none reported |
normal-binary |
aroA:CP4 (Act1) aroA:CP4 (e35S+ hsp intron) |
Jones et al. Plant Methods 2005 1:5 doi:10.1186/1746-4811-1-5 |
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