Table 1

Summary of main parameters reported for Agrobacterium-mediated transformation of wheat.
Wheat variety (S – spring) (W – winter)
 Explant type
 Embryo Axis removed
Agrobacterium strain (binary vector)
 Inoculation (Co-culture) *rt – room temp
 Control of Agrobacterium cells
 Plant selective agent
 Transformation Freq. (%)
 No of plants reported
 Refs
Bobwhite (S)
 IE (age NS*); 1–6 d PCIE; 10–25 d EC
 NS*
 C58-ABI (pMON18365)
 3 h, 23–25°C (2–3 d, 24–26°C)
 Carbenicillin (250 mg/l)
 G418
 1.4–4.3
 >100
 [17]
Bobwhite (S)
 4 d PCIE
 NS*
 C58-ABI (pMON30139 and others)
 15–30 min, 23–25°C (2–3 d, 23–25°C)
 Carbenicillin (250–500 mg/l)
 Glyphosate
 4.4
 3354
 [16]
Bobwhite (S)
 1–6 d PCIE; 8–30 d EC
 NS*
 C58-ABI (pMON18365)
 5–60 min, 23–26°C (2–3 d, 24–26°C)
 Carbenicillin (250 mg/l)
 G418
Paromomycin Glyphosate
 4.8–19
 154
 [18]
Bobwhite (S)
 3–6 PCIE
 NS*
 C58C1 (pPTN155)
 45 min – 3 h, 25°C (1–3 d, 25°C)
 Ticarcillium; Vancomycin Cefatoxin; (50 mg/l)
 G418
 0.5–1.5
 13
 [15]
Cadenza (S) Florida (W)
 0–72 h IE
 Yes
 AGL1 (pAL154/156)
 15 min-5 h, rt* (1–5 d, 24–25°C rt*)
 Timentin (160 mg/l)
 PPT (L-Phosphinothricin)
 0.3–3.3
 44
 [21]
Fielder (S)
 6–9 d PCIE
 Yes
 AGL0 (pBGX1)
 30–60 min rt* (2–3 d, 23–24°C)
 Timentin (150 mg/l)
 GFP, Bialaphos
 1.8
 4
 [19]
Veery-5 (S)
 14 d EC
 Yes
 LBA4404 (pHK21)
 15 min at rt* (1 d 27°C, 2 d 22°C)
 Timentin (150 mg/l)
 Glufosinate ammonium
 1.2–3.9
 17
 [20]
Vesna (S)
 IE (age NS*)
 NS*
 LBA4404 (pTOK233) AGL1 (pDM805)
 15–30 min, (3 d, 27°C)
 Cefotaxime (300 mg/l)
 PPT (L-Phosphinothricin)
 0.13–0.41
 6
 [45]
Various Chinese varieties (NS*)
 EC (age NS*)
 NS*
 AGL1 (pUNN-2)
 30–60 min (2 d, 28°C)
 Timentin (150 mg/l)
 Paromomycin
 3.7–5.9
 44
 [46]

IE – freshly isolated immature embryos; PCIE – pre-cultured immature embryos; EC – embryogenic callus; *NS not specified.

Jones et al. Plant Methods 2005 1:5   doi:10.1186/1746-4811-1-5