|
Detergents successfully employed for the solubilization of protein complexes prior to BN-PAGE. |
|||
| Detergent |
Sample |
Detergent concentration |
Reference |
|
|
|||
| Dodecylmaltoside |
Mammalian |
1.1% |
[1] |
| Mitochondria |
|||
| Plant Mitochondria |
1.5% |
[103] |
|
| Chloroplasts |
2.0% |
[75] |
|
| Yeast Peroxisomes |
0.5% and 1.0% |
[81] |
|
|
|
|||
| Digitonin |
Mammalian |
4 g/g protein |
[104] |
| Mitochondria |
|||
| Plant Mitochondria |
5.0 g/g protein |
[6] |
|
| Chloroplasts |
1.5 g/g protein |
[41] |
|
| Yeast Microsomes |
1.0% |
[80] |
|
| Yeast Peroxisomes |
1.0% |
[81] |
|
|
|
|||
| Triton X-100 |
Mammalian |
1.0, 1.4 and 2.4% |
[105] |
| Mitochondria |
|||
| Plant Mitochondria |
0.5% |
[6] |
|
| Yeast Peroxisomes |
0.2% and 1.0% |
[81] |
|
| Whole cellular lysates of different human cell lines |
0.1% |
[3] |
|
|
|
|||
| Brij 96 |
Whole cellular lysates of different human cell lines |
0.5% |
[3] |
|
|
|||
| Octylglucoside |
Tobacco microsomes |
40 mM |
[2] |
|
|
|||
| Saponin |
Whole cellular lysates of different human cell lines |
1.0% |
[3] |
Eubel et al. Plant Methods 2005 1:11 doi:10.1186/1746-4811-1-11 |
|||