Plant Methods
Volume 1
|
Viewing options:Associated material:Related literature:- Articles citing this article
- Other articles by authors
- Related articles/pages
Tools:Post to:
|
  ReviewBlue-native PAGE in plants: a tool in analysis of protein-protein interactionsHolger Eubel1  , Hans-Peter Braun2 and A Harvey Millar1  1ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Hwy, Crawley 6009, Perth, Australia 2Abteilung Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, 30419 Hannover, Germany  author email corresponding author email
Plant Methods 2005,
1:11doi:10.1186/1746-4811-1-11
|
|
| Published: |
16 November 2005 |
Abstract
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science. | 
on Google Scholar
